It has low magnifying power compared to TEM. In SEM sample is prepared by coating it with a thin layer of metal such as gold or palladium. SEM scans the surface of the objects and provides three-dimensional views of the surface structures. It uses scattered electrons as the light source. It is the most commonly used type of electron microscope. Its magnifying power is very high as compared to SEM and shows a 2-dimensional image. Tissues must be cut in thin sections for viewing under TEM. It provides a detailed view of the internal structure of the prepared test samples. Transmission Electron Microscope ( TEM)Īs the name suggests, this type uses transmitted electrons as light sources. There are two types of electron microscopes, with different operating styles: the transmission electron microscope (TEM) and the scanning electron microscope (SEM). In electron microscope, the entire illuminating imaging system is usually referred to as the microscope column and is constructed upside down as compared to a light microscope.
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Image viewing and recording system: The magnified image of the specimen is formed as a photograph (called an electron micrograph) or as an image on a TV screen.In an electron microscope, the coils bend the electron beams the same way. In an ordinary microscope, the glass lenses bend (or refract) the light beams passing through them to produce magnification.
The magnetic lens of an electron microscope can have different powers (focal lengths and magnification) depending on the amount of current flowing through the electrical coils. In addition to the condenser and objective lenses, a third projector lens is also present. There are 3 sets of electromagnetic lenses in an electron microscope as compared to two in light microscopes.
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Electromagnetic fields (instead of lenses): The lenses that make the specimen seem bigger are replaced by a series of coil-shaped electromagnets through which the electron beam travels also known as electromagnetic lenses.
These electron beams are obtained when the tungsten filament in an electron microscope, is heated by applying a high voltage current, which is used as a light beam.
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